Composite

Part:BBa_K4757006

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-11-16)


pEM7-XylS-K38R-L224Q/Pm

Expression cassette with the regulatory transcription factor XylS-K38R-L224Q, capable of sensing TPA. The XylS-mt expression is controlled by the constitutive promoter pEM7 and upon TPA binding XylS-wt binds to Pm and activates gene Expression. For more information visit our composite part page BBa_K4757999

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 80
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 802
  • 1000
    COMPATIBLE WITH RFC[1000]


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